Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,122,267	A→C	S35A (TCG→GCG)	wcaF ←	colanic acid biosynthesis acetyltransferase WcaF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,122,267	0	A	C	100.0%	38.4 / NA	13	S35A (TCG→GCG)	wcaF	colanic acid biosynthesis acetyltransferase WcaF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (4/9);  total (4/9)

TAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAG  >  NZ_CP009273/2122192‑2122349                                                                            |                                                                                   taccTACGTTTTTTCCTATTTTTGCCCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATAttg                                                                      <  1:181410/87‑1 (MQ=255) tAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATAttg                                                                      <  1:147354/90‑1 (MQ=255) tAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATAttg                                                                      <  2:248447/90‑1 (MQ=255)           ttttCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATATTGTTGcc                                                                 >  1:312577/1‑85 (MQ=255)           ttttCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATATTGTTGcc                                                                 <  2:312577/85‑1 (MQ=255)           ttttCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATATTGTTGCCTGTAc                                                            <  2:232232/90‑1 (MQ=255)                           ccGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGc                                            <  2:389412/90‑1 (MQ=255)                             gAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCAc                                          <  1:165311/90‑1 (MQ=255)                                       aataaAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATAttg                                                                      <  1:300679/52‑1 (MQ=255)                                       aataaAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATAttg                                                                      >  2:300679/1‑52 (MQ=255)                                       aataaAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCg                                >  1:80151/1‑90 (MQ=255)                                             aaaGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTgccg                          <  2:80151/90‑1 (MQ=255)                                                                     tgtgGCGCCCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCgag  >  2:316474/1‑90 (MQ=255)                                                                            |                                                                                   TAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAG  >  NZ_CP009273/2122192‑2122349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑