Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,202,171	G→C	W242C (TGG→TGC)	yehP →	VWA domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,202,171	0	G	C	100.0%	39.8 / NA	13	W242C (TGG→TGC)	yehP	VWA domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (8/5);  total (8/5)

CTGGTTGATCAAAGCGGATCGATGGTCGATTCGGTGATCCACTCTGCGGTGATGGCGGCCTGTTTGTGGCAGTTACCCGGCATTCGTACCCATCTGGTGGCGTTTGACACAAGCGTCGTTGATCTCACGGCAGACGTTGCCGATCCGG  >  NZ_CP009273/2202103‑2202250                                                                     |                                                                                cTGGTTGATCAAAGCGGATCGATGGTCGATTCGGTGATCCACTCTGCGGTGATGGCGGCCTGTTTGTGCCAGTTACCCGGCATTCGTAcc                                                            <  2:105493/90‑1 (MQ=255)          cAAAGCGGATCGATGGTCGATTCGGTGATCCACTCTGCGGTGATGGCGGCCTGTTTGTGCCAGTTACCCGGCATTCGTACCCATCtggtg                                                   <  2:360290/90‑1 (MQ=255)           aaaGCGGATCGATGGTCGATTCGGTGATCCACTCTGCGGTGATGGCGGCCTGTTTGTGCCAGTTACCCGGCATTCGTACCCATCtggtgg                                                  >  1:72417/1‑90 (MQ=255)                                cGGTGATCCACTCTGCGGTGATGGCGGCCTGTTTGTGCCAGTTACCCGGCATTCGTACCCATCTGGTGGCGTTTGACACAAGCGTCGTTg                             >  2:242781/1‑90 (MQ=255)                                  gTGATCCACTCTGCGGTGATGGCGGCCTGTTTGTGCCAGTTACCCGGCATTCGTACCCATCTGGTGGCGTTTGACACAAGCGTCGTTGAt                           >  2:165622/1‑90 (MQ=255)                                       ccACTCTGCGGTGATGGCGGCCTGTTTGTGCCAGTTACCCg                                                                       >  1:393496/1‑41 (MQ=255)                                       ccACTCTGCGGTGATGGCGGCCTGTTTGTGCCAGTTACCCg                                                                       <  2:393496/41‑1 (MQ=255)                                           tctGCGGTGATGGCGGCCTGTTTGTGCCAGTTACCCGGCATTCGTACCCATCTGGTGGCGTTTGACACAAGCGTCGTTGATCTCACGGCa                  >  2:164587/1‑90 (MQ=255)                                              gCGGTGATGGCGGCCTGTTTGTGCCAGTTACCCGGCATTCGTACCCATCTGGTGGCGTTTGACACAAGCGTCGTTGATCTCACGGCAGAc               <  2:144999/90‑1 (MQ=255)                                                    aTGGCGGCCTGTTTGTGCCAGTTACCCGGCATTCGTACCCATCTGGTGGCGTTTGACACAAGCGTCGTTGATCTCACGGCAGACGTTGcc         >  1:111710/1‑90 (MQ=255)                                                      ggcggcCTGTTTGTGCCAGTTACCCGGCATTCGTACCCATCTGGTGGCGTTTGACACAAGCGTCGTTGATCTCACGGCAGACGTTGCCGa       <  2:209651/90‑1 (MQ=255)                                                        cggcCTGTTTTTGCCAGTTACCCTGCATTCGTAACCATCTGGTGGCGTTTGGCACCAGCGTCGTTGATCTCACGGCAGAAGTTGCCGATc     >  1:282578/1‑90 (MQ=255)                                                           ccTGTTTGTGCCAGTTACCCGGCATTCGTACCCATCTGGTGGCGTTTGACACAAGCGTCGTTGATCTCACGGCAGACGTTGCCGATCCgg  >  2:217698/1‑90 (MQ=255)                                                                     |                                                                                CTGGTTGATCAAAGCGGATCGATGGTCGATTCGGTGATCCACTCTGCGGTGATGGCGGCCTGTTTGTGGCAGTTACCCGGCATTCGTACCCATCTGGTGGCGTTTGACACAAGCGTCGTTGATCTCACGGCAGACGTTGCCGATCCGG  >  NZ_CP009273/2202103‑2202250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑