Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,505,777	T→C	I137V (ATC→GTC)	mntH ←	Nramp family divalent metal transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,505,777	0	T	C	100.0%	45.6 / NA	15	I137V (ATC→GTC)	mntH	Nramp family divalent metal transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/8);  total (7/8)

CGGCAACAAACAACAGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGC  >  NZ_CP009273/2505690‑2505840                                                                                        |                                                                cgGCAACAAACAACAGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGAccc                                                               >  1:157154/1‑90 (MQ=255)                       gCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGcaa                                         >  1:278249/1‑90 (MQ=255)                       gCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGcaa                                         >  2:75783/1‑90 (MQ=255)                        ccAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGcaac                                        >  2:257346/1‑90 (MQ=255)                        ccAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGcaac                                        <  2:264238/90‑1 (MQ=255)                              aCTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGaa                                  <  1:208236/90‑1 (MQ=255)                              aCTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGaa                                  <  1:245550/90‑1 (MQ=255)                                        gCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAGAGTCGCGACCCCCGTCAGCACCTCGCCCTGCAACAACGAAACACCAAGaa                        >  2:367592/1‑90 (MQ=255)                                        gCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGaa                        >  2:95632/1‑90 (MQ=255)                                          ggTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATg                      <  2:21241/90‑1 (MQ=255)                                          ggTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATg                      <  2:284067/90‑1 (MQ=255)                                                     gcgACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAAcc           >  2:32412/1‑90 (MQ=255)                                                           ttGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGaa                                  <  1:105781/61‑1 (MQ=255)                                                           ttGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGaa                                  >  2:105781/1‑61 (MQ=255)                                                            tGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATcgcc    <  2:157154/90‑1 (MQ=255)                                                              cagcaTTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATcgccgc  <  1:75783/90‑1 (MQ=255)                                                                                        |                                                                CGGCAACAAACAACAGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGC  >  NZ_CP009273/2505690‑2505840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑