Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,882,413	A→C	V349G (GTT→GGT)	cysI ←	assimilatory sulfite reductase (NADPH) hemoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,882,413	0	A	C	100.0%	29.1 / NA	10	V349G (GTT→GGT)	cysI	assimilatory sulfite reductase (NADPH) hemoprotein subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (3/7);  total (3/7)

CCGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGC  >  NZ_CP009273/2882326‑2882498                                                                                        |                                                                                      ccGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAccc                                                                                     >  2:183101/1‑90 (MQ=255)                       gATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTc                                                               <  1:83995/90‑1 (MQ=255)                               aGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAAc                                                       <  2:107956/90‑1 (MQ=255)                               aGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAAc                                                       <  2:280665/90‑1 (MQ=255)                               aGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAAc                                                       <  2:302713/90‑1 (MQ=255)                                ggATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACt                                                      <  1:183101/90‑1 (MQ=255)                                 gATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTc                                                     <  1:133159/90‑1 (MQ=255)                                     cGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCatat                                                 >  1:294691/1‑90 (MQ=255)                                           ttttCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACgg                                           >  1:278150/1‑90 (MQ=255)                                                                       tATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGAc               <  2:278150/90‑1 (MQ=255)                                                                                    cTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGc  >  1:213265/1‑90 (MQ=255)                                                                                        |                                                                                      CCGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGC  >  NZ_CP009273/2882326‑2882498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑