Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,113,649	T→G	K330N (AAA→AAC)	glcA ←	glycolate permease GlcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,113,649	0	T	G	100.0%	52.6 / NA	17	K330N (AAA→AAC)	glcA	glycolate permease GlcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (4/13);  total (4/13)

CACTTGTTGATGCAAATGAGGGATCTGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCACCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGACTATA  >  NZ_CP009273/3113574‑3113735                                                                            |                                                                                       cacTTGTTGATGCAAATGAGGGATCTGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCGTTAAACGGCTTCAt                                                                          <  1:288347/90‑1 (MQ=255)  acTTGTTGATGCAAATGAGGGATCTGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCGTTAAACGGCTTCATg                                                                         <  2:70248/90‑1 (MQ=255)  acTTGTTGATGCAAATGAGGGATCTGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCGTTAAACGGCTTCATg                                                                         <  2:250547/90‑1 (MQ=255)  acTTGTTGATGCAAATGAGGGATCTGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCGTTAAACGGCTTCATg                                                                         <  1:370914/90‑1 (MQ=255)   cTTGTTGATGCAAATGAGGGATCTGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCGTTAAACGGCTTCATgg                                                                        >  1:60807/1‑90 (MQ=255)       ttgATGCAAATGAGGGATCTGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCGTTAAACGGCTTCATGGTCCa                                                                    <  1:47801/90‑1 (MQ=255)            gCAAATGAGGGATCTGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCGTTAAACGGCTTCATGGTCCAGATgg                                                               <  2:20562/90‑1 (MQ=255)                                  aaccACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCGTTAAACGGCTTCATGGTCCAGATGGTGACCAGCACCGTTAAGATTaa                                         <  1:345599/87‑1 (MQ=255)                                                               gAGCAAATAACGCGTTAAACGGCTTCATGGTCCAGATgg                                                               >  2:93624/1‑39 (MQ=39)                                                               gAGCAAATAACGCGTTAAACGGCTTCATGGTCCAGATgg                                                               <  1:93624/39‑1 (MQ=255)                                                               gAGCAAATAACGCGTTAAACGGCTTCATGGTCCAGATGGTGACCAGCACCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGcc            >  1:110734/1‑90 (MQ=255)                                                                aGCAAATAACGCGTTAAACGGCTTCATGGTCCAGATGGTGACCAGCACCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGccc           <  2:328504/90‑1 (MQ=255)                                                                  cAAATAACGCGTTAAACGGCTTCATGGTCCAGATGGTGACCAGCACCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCa         <  1:363012/90‑1 (MQ=255)                                                                    aaTAACGCGTTAAACGGCTTCATGGTCCAGATGGTGACCAGCACCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGa       >  1:297886/1‑90 (MQ=255)                                                                     aTAACGCGTTAAACGGCTTCATGGTCCAGATGGTGACCAGCACCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGAc      <  1:256322/90‑1 (MQ=255)                                                                       aaCGCGTTAAACGGCTTCATGGTCCAGATGGTGACCAGCACCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGACta    <  1:82510/90‑1 (MQ=255)                                                                       aaCGCGTTAAACGGCTTCATGGTCCAGATGGTGACCAGCACCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGACta    <  2:45794/90‑1 (MQ=255)                                                                         cgcgTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGACtata  >  1:373029/1‑90 (MQ=255)                                                                            |                                                                                       CACTTGTTGATGCAAATGAGGGATCTGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCACCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGACTATA  >  NZ_CP009273/3113574‑3113735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑