Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,717,969	T→C	E237G (GAA→GGA)	glyQ ←	glycine‑‑tRNA ligase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,717,969	0	T	C	100.0%	34.9 / NA	12	E237G (GAA→GGA)	glyQ	glycine‑‑tRNA ligase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/5);  total (7/5)

GTTGAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGTCCACATCCGCG  >  NZ_CP009273/3717911‑3718040                                                           |                                                                        gTTGAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTtctc                                          >  2:101797/1‑90 (MQ=255)          gtgGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTc                                 >  2:310321/1‑90 (MQ=255)           tgGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCg                                >  2:350492/1‑90 (MQ=255)                           tACGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGa                <  1:101797/90‑1 (MQ=255)                           tACGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGa                <  1:278353/90‑1 (MQ=255)                           tACGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGa                <  1:46337/90‑1 (MQ=255)                           tACGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGa                <  1:64368/90‑1 (MQ=255)                                  gTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGTCcac         >  1:22312/1‑90 (MQ=255)                                       cTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGTCCACATCcg    >  1:131202/1‑90 (MQ=255)                                         ggcagcggcagcGGATTTCCCAGCGCCAGCAGCTGCTGCGcttc                                                <  1:308827/44‑1 (MQ=255)                                         ggcagcggcagcGGATTTCCCAGCGCCAGCAGCTGCTGCGcttc                                                >  2:308827/1‑44 (MQ=255)                                         ggcagcggcagcGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGTCCACATCcgcg  >  2:85392/1‑90 (MQ=255)                                                           |                                                                        GTTGAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGTCCACATCCGCG  >  NZ_CP009273/3717911‑3718040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑