Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,786,467	T→C	I193V (ATT→GTT)	yibB ←	protein YibB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,786,467	0	T	C	100.0%	49.5 / NA	16	I193V (ATT→GTT)	yibB	protein YibB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/4);  total (12/4)

TCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAAT  >  NZ_CP009273/3786406‑3786549                                                              |                                                                                   tCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGAt                                                        <  1:55445/90‑1 (MQ=255)     tCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGa                                                    <  1:57601/90‑1 (MQ=255)                tgtgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCt                                         >  2:206923/1‑90 (MQ=255)                             ttGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGa                            >  2:362428/1‑90 (MQ=255)                                              tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt           >  2:200200/1‑90 (MQ=255)                                                taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGAtgtg                                                     <  1:316874/46‑1 (MQ=255)                                                taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGAtgtg                                                     >  2:316874/1‑46 (MQ=255)                                                  cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcat       >  2:23559/1‑90 (MQ=255)                                                  cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcat       >  2:267997/1‑90 (MQ=255)                                                  cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcat       >  2:316286/1‑90 (MQ=255)                                                  cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcat       >  2:59467/1‑90 (MQ=255)                                                     gATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCaa    >  1:15412/1‑90 (MQ=255)                                                     gATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCaa    >  2:189519/1‑90 (MQ=255)                                                       ttGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAAt  >  1:174712/1‑90 (MQ=255)                                                         gCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGaaa                          <  1:160397/64‑1 (MQ=255)                                                         gCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGaaa                          >  2:160397/1‑64 (MQ=255)                                                              |                                                                                   TCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAAT  >  NZ_CP009273/3786406‑3786549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑