Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,034,572	T→G	Q74H (CAA→CAC)	mobB ←	molybdopterin‑guanine dinucleotide biosynthesis protein MobB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,034,572	0	T	G	100.0%	67.4 / NA	22	Q74H (CAA→CAC)	mobB	molybdopterin‑guanine dinucleotide biosynthesis protein MobB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/14);  total (8/14)

TATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCA  >  NZ_CP009273/4034499‑4034656                                                                          |                                                                                     tATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGa                                                                      <  1:131108/90‑1 (MQ=255)          gACTTGCGAGAAACTGTAGACCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTtgtg                                                             >  2:255655/1‑90 (MQ=255)            cTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGcc                                                           <  2:72874/90‑1 (MQ=255)                   gaAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAg                                                    >  2:157607/1‑90 (MQ=255)                         gTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTgc                                              >  2:165076/1‑90 (MQ=255)                           aGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTgcgc                                            <  1:3860/90‑1 (MQ=255)                           aGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTgcgc                                            <  2:100021/90‑1 (MQ=255)                           aGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTgcgc                                            <  1:157607/90‑1 (MQ=255)                           aGACCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTgcgc                                            <  1:255655/90‑1 (MQ=255)                           aGACCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTgcgc                                            <  2:305235/90‑1 (MQ=255)                                 aGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCa                                      >  1:63549/1‑90 (MQ=255)                                 aGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCa                                      >  2:372526/1‑90 (MQ=255)                                       tcttcGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCt                                 >  1:71674/1‑89 (MQ=255)                                       tcttcGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCt                                 <  2:71674/89‑1 (MQ=255)                                                 gtgtTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTg                      <  1:257021/90‑1 (MQ=255)                                                         gTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCCGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCa              <  1:31658/90‑1 (MQ=255)                                                           catcaAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAAc            >  1:152873/1‑90 (MQ=255)                                                              caAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATc         <  2:152873/90‑1 (MQ=255)                                                                aGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCa       <  2:305270/90‑1 (MQ=255)                                                                aGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCa       <  2:300160/90‑1 (MQ=255)                                                                  gCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCata     <  2:370392/90‑1 (MQ=255)                                                                     cATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCa  >  2:51837/1‑90 (MQ=255)                                                                          |                                                                                     TATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCA  >  NZ_CP009273/4034499‑4034656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑