Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	502,414	T→C	D42G (GAT→GGT)	ybaK ←	Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase YbaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	502,414	0	T	C	100.0%	13.3 / NA	6	D42G (GAT→GGT)	ybaK	Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase YbaK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/4);  total (2/4)

TCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATGGATTTGAAACGA  >  NZ_CP009273/502327‑502499                                                                                        |                                                                                      tCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGAccc                                                                                     >  2:300223/1‑90 (MQ=255)                 ccGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGACCCGGATTCAAACCTAAtt                                                                     <  2:105426/90‑1 (MQ=255)                                                      gTTCACTGCCACCAGCAGCGTTTTGTAGACCTGACCCGGATTCa                                                                              >  1:271856/1‑44 (MQ=255)                                                      gTTCACTGCCACCAGCAGCGTTTTGTAGACCTGACCCGGATTCa                                                                              <  2:271856/44‑1 (MQ=255)                                                                               tAGACCTGACCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATGGATTTGa       <  2:174951/90‑1 (MQ=255)                                                                                 gACCTGACCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATGGATTTGaaa     >  1:950/1‑90 (MQ=255)                                                                                    cTGACCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCTAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATGGATTTGAAACGa  <  2:301714/90‑1 (MQ=255)                                                                                        |                                                                                      TCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATGGATTTGAAACGA  >  NZ_CP009273/502327‑502499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑