Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	674,442	T→G	T141P (ACT→CCT)	ybeT ←	tetratricopeptide repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	674,442	0	T	G	100.0%	46.3 / NA	15	T141P (ACT→CCT)	ybeT	tetratricopeptide repeat protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/7);  total (8/7)

TTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTG  >  NZ_CP009273/674360‑674530                                                                                   |                                                                                         ttGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATtga                                                                                   <  1:396368/90‑1 (MQ=255)           gTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTgagcatgagcat                                                                         <  2:175449/90‑1 (MQ=255)           gTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTgagcatgagcat                                                                         <  2:385647/90‑1 (MQ=255)                     tttttGAGATTCATAATGGTTAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTc                                                               >  2:257577/1‑90 (MQ=255)                               tCATAATGGTTAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTcc                                                     >  1:2640/1‑90 (MQ=255)                                cATAATGGTTAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCt                                                    <  2:390778/90‑1 (MQ=255)                                         tAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCaga                                           >  2:31322/1‑90 (MQ=255)                                          aaCATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCagag                                          >  1:387008/1‑90 (MQ=255)                                              ttAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAAtt                                      <  1:265727/90‑1 (MQ=255)                                                aaTACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGc                                    >  1:125907/1‑90 (MQ=255)                                                      tGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTACACCAGAGAATTGCTTTACg                              >  1:132395/1‑90 (MQ=255)                                                                  gTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGttt                  >  1:287291/1‑90 (MQ=255)                                                                       tCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGAccc             >  1:242337/1‑90 (MQ=255)                                                                           aCCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAg         <  2:2640/90‑1 (MQ=255)                                                                                aaGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGA‑‑CCCAGACCTTTg  <  2:223298/90‑1 (MQ=255)                                                                                   |                                                                                         TTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTG  >  NZ_CP009273/674360‑674530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑