Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	818,875	A→G	N107N (AAT→AAC)	clsB ←	cardiolipin synthase ClsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	818,875	0	A	G	100.0%	28.4 / NA	10	N107N (AAT→AAC)	clsB	cardiolipin synthase ClsB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/4);  total (6/4)

TATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACA  >  NZ_CP009273/818790‑818953                                                                                      |                                                                               tatGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTgg                                                                            >  2:100883/1‑90 (MQ=255)            gAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCa                                                                 >  2:139725/1‑90 (MQ=255)                           ccAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCgg                                                  <  1:129612/90‑1 (MQ=255)                             aaTAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCgggg                                                <  1:100883/90‑1 (MQ=255)                                       ataCGCGCGTCGGTCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGGGCGCATACCAAAAAGGCGAGGGCGGGGATCGtagtag                                      >  1:316002/1‑90 (MQ=255)                                       ataCGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGtagtag                                      >  1:256554/1‑90 (MQ=255)                                             gcgTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAac                                >  1:302465/1‑90 (MQ=255)                                                                      gCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCTGAACACTACGCCAGCTGCCGTCAGTTCAt       >  1:31250/1‑90 (MQ=255)                                                                      gCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCAt       <  2:137043/90‑1 (MQ=255)                                                                           cGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCTGAACACTACGCCAGCTGCCGTCAGTTCATTGACa  <  2:31250/90‑1 (MQ=255)                                                                                      |                                                                               TATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACA  >  NZ_CP009273/818790‑818953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑