Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	951,809	T→G	T94P (ACC→CCC)	ycaO ←	30S ribosomal protein S12 methylthiotransferase accessory factor YcaO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	951,809	0	T	G	100.0%	18.1 / NA	7	T94P (ACC→CCC)	ycaO	30S ribosomal protein S12 methylthiotransferase accessory factor YcaO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (4/3);  total (4/3)

AGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGTTTCGCCCAGCCAGAAGTCCGCAAAAAAGTAGTTGGTTGAGAGACGCTCGAAATATTCACCGAGTGCAGAAGCCAGCGC  >  NZ_CP009273/951727‑951887                                                                                   |                                                                               agcCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGGTTCGccc                                                                         >  1:267032/1‑90 (MQ=255)                           ggTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGGTTCGCCCAGCCAGAAGTCCGCAAAAAAGTAgtt                                               <  2:267032/90‑1 (MQ=255)                            gTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGGTTCGCCCAGCCAGAAGTCCGCAAAAAAGTAgttg                                              >  2:374696/1‑90 (MQ=255)                                     aaCCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGGTTCGCCCAGCCAGAAGTCCGCAAAAAAGTAGTTGGTTgagaga                                     <  1:140368/90‑1 (MQ=255)                                                       tAATGCACGAACGGACCGTTGGCGATGGGTTCGCCCAGCCAGAAGTCCGCAAAAAAGTAGTTGGTTGAGAGACGCTCGAAATATTCACCg                   <  2:108321/90‑1 (MQ=255)                                                                   ggACCGTTGGCGATGGGTTCGCCCAGCCAGAAGTCCGCAAAAAAGTAGTTGGTTGAGAGACGCTCGAAATATTCACCGAGTGCAGAAGcc       >  1:168420/1‑90 (MQ=255)                                                                        gTTGGCGATGGGTTCGCCCAGCCAGAAGTCCGCAAAAAAGTAGTTGGTTGAGAGACGCTCGAAATATTCACCGAGTGCAGAAGCCAgcgc  >  2:109416/1‑90 (MQ=255)                                                                                   |                                                                               AGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGTTTCGCCCAGCCAGAAGTCCGCAAAAAAGTAGTTGGTTGAGAGACGCTCGAAATATTCACCGAGTGCAGAAGCCAGCGC  >  NZ_CP009273/951727‑951887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑