Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,062,210	T→C	G390G (GGT→GGC)	agp →	bifunctional glucose‑1‑phosphatase/inositol phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,062,210	0	T	C	100.0%	38.7 / NA	13	G390G (GGT→GGC)	agp	bifunctional glucose‑1‑phosphatase/inositol phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/6);  total (7/6)

GTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAG  >  NZ_CP009273/1062124‑1062285                                                                                       |                                                                            gtgtATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGc                                                                          >  2:65376/1‑90 (MQ=255)  tgtATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGcc                                                                         <  1:244809/90‑1 (MQ=255)  tgtATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGcc                                                                         <  2:271668/90‑1 (MQ=255)          agTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGAc                                                                 <  1:62344/90‑1 (MQ=255)           gTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACg                                                                >  1:237510/1‑90 (MQ=255)                               gCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATg                                            >  1:144131/1‑90 (MQ=255)                                   aTGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                        <  2:237510/90‑1 (MQ=255)                                                      aCCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                        <  1:131771/71‑1 (MQ=255)                                                      aCCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                        >  2:131771/1‑71 (MQ=255)                                                                 gtgtGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGa          >  1:32252/1‑90 (MQ=255)                                                                 gtgtGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGa          <  2:267468/90‑1 (MQ=255)                                                                 gtgtGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGa          >  2:338169/1‑90 (MQ=255)                                                                         cTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAg  >  2:36176/1‑90 (MQ=255)                                                                                       |                                                                            GTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAG  >  NZ_CP009273/1062124‑1062285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑