| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,065,426 | T→G | T129P (ACC→CCC) | rutF ← | pyrimidine utilization flavin reductase protein F |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,065,426 | 0 | T | G | 100.0% | 10.9 / NA | 5 | T129P (ACC→CCC) | rutF | pyrimidine utilization flavin reductase protein F |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (4/1); total (4/1) | |||||||||||
GTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCC > NZ_CP009273/1065375‑1065503 | gtgtggtgtgACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACg > 1:348338/1‑90 (MQ=255) gCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTCaccacc < 1:348143/48‑1 (MQ=255) gCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTCaccacc > 2:348143/1‑48 (MQ=255) gcgcAAAACAGAATGTCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAGTTGCGGGCAt > 1:97670/1‑90 (MQ=255) gAATGTCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAGTTGCGGGCATCCGGTCAccc > 1:17333/1‑90 (MQ=255) | GTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCC > NZ_CP009273/1065375‑1065503 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |