Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,153,703	A→T	I127F (ATC→TTC)	ptsG →	PTS glucose transporter subunit IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,153,703	0	A	T	100.0%	33.3 / NA	10	I127F (ATC→TTC)	ptsG	PTS glucose transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (8/2);  total (8/2)

TGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGA  >  NZ_CP009273/1153620‑1153787                                                                                    |                                                                                     tGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAg                                                                                <  1:401779/90‑1 (MQ=255)             tACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTa                                                                    >  1:133234/1‑90 (MQ=255)                  gctgAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGt                                                               >  1:174541/1‑90 (MQ=255)                  gctgAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGt                                                               >  1:250225/1‑90 (MQ=255)                        gaaATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTAc                                                         <  1:336531/90‑1 (MQ=255)                            tCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGGGGGGTTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTa                                                     >  1:204577/1‑90 (MQ=255)                                           tGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTGCATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGt                                      >  1:242606/1‑90 (MQ=255)                                            ggCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTa                                     >  2:273141/1‑90 (MQ=255)                                                           aCTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGc                      >  2:339714/1‑90 (MQ=255)                                                                               gTGCGTTCGCAGCGTACATGTTTAACCGTTCCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGa  >  1:10424/1‑90 (MQ=255)                                                                                    |                                                                                     TGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGA  >  NZ_CP009273/1153620‑1153787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑