Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,230,259	C→T	intergenic (‑86/‑135)	nhaB ← / → fadR	Na(+)/H(+) antiporter NhaB/fatty acid metabolism transcriptional regulator FadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,230,259	0	C	T	100.0%	21.1 / NA	7	intergenic (‑86/‑135)	nhaB/fadR	Na(+)/H(+) antiporter NhaB/fatty acid metabolism transcriptional regulator FadR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/3);  total (4/3)

AATCAATGATGTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTT  >  NZ_CP009273/1230198‑1230338                                                              |                                                                                aatCAATGATGTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTTTTGATAGTCAAGGGGAAAGAGATGCGGa                                                     <  1:192989/90‑1 (MQ=255)    cAATGATGTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGGGAGGCAAGTTTTGATAGTCAAGGGGAAAGAGATGCGGaaaa                                                  >  2:78908/1‑90 (MQ=255)                                        gTCAGGCAGGAGTGAGGCAAGTTTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCt                 <  1:7401/87‑1 (MQ=255)                                        gTCAGGCAGGAGTGAGGCAAGTTTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCt                 >  2:7401/1‑87 (MQ=255)                                          caggcaggAGTGAGGCAAGTTTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTAt            >  2:321976/1‑90 (MQ=255)                                                    tGAGGCAAGTTTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGtt  >  1:2919/1‑90 (MQ=255)                                                           aGTTTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCg       <  2:178544/78‑1 (MQ=255)                                                              |                                                                                AATCAATGATGTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTT  >  NZ_CP009273/1230198‑1230338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑