Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,499,566	A→G	S258P (TCC→CCC)	ydcO ←	BenE family transporter YdcO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,499,566	0	A	G	100.0%	21.5 / NA	8	S258P (TCC→CCC)	ydcO	BenE family transporter YdcO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/4);  total (4/4)

GTTGATCTTTATCCGGATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGAAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAGCTGCTTTCATTGCTGCGATA  >  NZ_CP009273/1499479‑1499648                                                                                        |                                                                                   gTTGATCTTTATCCGGATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGgaa                                                                                  <  2:146961/90‑1 (MQ=255)    gATCTTTATCCGGATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGgaaaaa                                                                               >  1:160795/1‑90 (MQ=255)     aTCTTTATCCGGATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAAc                                                                              >  1:102676/1‑90 (MQ=255)                gATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCa                                                                   >  1:1803/1‑90 (MQ=255)                     gcTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCa                                                              >  2:19622/1‑90 (MQ=255)                        tCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTa                                                           <  2:1803/90‑1 (MQ=255)                            ggCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATa                                                       <  1:101646/90‑1 (MQ=255)                                                                   cGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAGCTGCtt                <  1:19622/90‑1 (MQ=255)                                                                          aaCGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAGCTGCTTTCATtgc         >  2:356369/1‑90 (MQ=255)                                                                                 aaaGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAGCTGCTTTCATTGCTGCGATa  <  2:47047/90‑1 (MQ=255)                                                                                        |                                                                                   GTTGATCTTTATCCGGATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGAAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAGCTGCTTTCATTGCTGCGATA  >  NZ_CP009273/1499479‑1499648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑