Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,659,576	T→C	F14S (TTC→TCC)	clcB →	voltage‑gated ClC‑type chloride channel ClcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,659,576	0	T	C	100.0%	35.5 / NA	12	F14S (TTC→TCC)	clcB	voltage‑gated ClC‑type chloride channel ClcB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/5);  total (7/5)

GCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTTCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATGCTGCTACT  >  NZ_CP009273/1659508‑1659657                                                                     |                                                                                  gCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAAc                                                              <  1:329328/90‑1 (MQ=255)     gtgtTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAgtc                                                          >  1:221670/1‑90 (MQ=255)          tCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCgg                                                     >  1:326918/1‑90 (MQ=255)                              tGCATCATCTACACATCTATCCGGATCTGCGCACCATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTttgccg                                 >  2:351384/1‑90 (MQ=255)                               gCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTttgccgt                                <  1:351776/90‑1 (MQ=255)                                   catcTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTttgccgttgcc                            >  2:129776/1‑90 (MQ=255)                                        acacATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGtt                       >  1:401851/1‑90 (MQ=255)                                           caTCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCg                    >  1:103206/1‑90 (MQ=255)                                            aTCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGt                   <  2:189721/90‑1 (MQ=255)                                              cTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCa                 <  1:129776/90‑1 (MQ=255)                                                          gcgcACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATgctgct     >  2:315463/1‑90 (MQ=255)                                                             cACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATGCTGCTACt  <  1:315463/90‑1 (MQ=255)                                                                     |                                                                                  GCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTTCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATGCTGCTACT  >  NZ_CP009273/1659508‑1659657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑