Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,838,813	T→C	I106V (ATT→GTT)	ynjI ←	DUF1266 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,838,813	0	T	C	90.9%	26.5 / ‑4.7	11	I106V (ATT→GTT)	ynjI	DUF1266 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (6/4);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGACCATTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCATAATTAATAGAT  >  NZ_CP009273/1838736‑1838896                                                                              |                                                                                    aGACCATTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTg                                                                         <  2:276143/90‑1 (MQ=255)      aTTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGataat                                                                    <  1:172890/90‑1 (MQ=255)      aTTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGataat                                                                    <  2:218042/90‑1 (MQ=255)       ttGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAATACCTTTAGCCTGataata                                                                   >  1:16515/1‑90 (MQ=255)       ttGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGataata                                                                   >  1:48726/1‑90 (MQ=255)                                               gcCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTa                                                                              <  1:55039/39‑1 (MQ=255)                                               gcCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTa                                                                              >  2:55039/1‑39 (MQ=39)                                               gcCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACa                           >  1:173995/1‑90 (MQ=255)                                                                 cTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCAtaatt         >  2:184058/1‑90 (MQ=255)                                                                   gAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCAtaattaa       >  2:256178/1‑90 (MQ=255)                                                                        gCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCATAATTAATAGAt  >  1:89144/1‑90 (MQ=255)                                                                              |                                                                                    AGACCATTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCATAATTAATAGAT  >  NZ_CP009273/1838736‑1838896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑