| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,417,788 | (C)6→7 | coding (69/120 nt) | BW25113_RS26130 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,417,782 | 1 | . | C | 88.9% | 21.0 / ‑2.9 | 9 | coding (75/120 nt) | BW25113_RS26130 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (5/3); total (5/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑CCT‑CCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGAAG > NZ_CP009273/1417697‑1417863 | cGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑CCT‑cccc > 1:93896/1‑90 (MQ=33) gTCTGTCAGTCAGTGCGTGAAGCCGCCACCGCCTCCGGCGTGGATAATGCAG‑CCT‑cccccccg < 2:282715/63‑3 (MQ=255) gTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑CCT‑cccccccg > 1:282715/1‑61 (MQ=25) cagtGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTc > 1:497253/1‑90 (MQ=255) gCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCggg > 2:184188/1‑90 (MQ=255) tGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAAc < 2:523356/90‑1 (MQ=255) gCGTGGATAATGCAG‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGcc > 1:494997/1‑90 (MQ=255) cGTGGATAATGCAG‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGccc < 2:50994/90‑1 (MQ=255) cGTGGATAATGCAG‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGccc < 2:93896/90‑1 (MQ=255) aTGCAGCCCC‑CCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGTTAATGATGCAGGCCCAACTTGaa < 2:551722/90‑1 (MQ=25) aTGCAG‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGaa > 1:150676/1‑90 (MQ=255) tGCAG‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGAAg > 2:229341/1‑90 (MQ=255) | CGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑CCT‑CCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGAAG > NZ_CP009273/1417697‑1417863 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |