Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927368,0270AG54.5% 6.1 / 11.2 11I64V (ATT→GTT) yabIDedA family protein
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base G (6/0);  total (9/2)
Fisher's exact test for biased strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCT‑GGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATT  >  NZ_CP009273/67949‑68106
                                                                               |                                                                               
ggTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTTTGGCACGCCTGGCT‑GGCAGGGGTTATTGgctgc                                                                      >  2:497725/1‑90 (MQ=255)
      gTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGGTTAGGTTTTGGGACGCCTGGCTGGGCGGGGGTTTTTGGTTGTTTgtg                                                                 >  1:373280/1‑88 (MQ=255)
      gTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGGTAAGTTTTTGGGACGCCCGGCC‑GGGGGGGGTTTTTGGGTGGTTGGTg                                                                >  1:130353/1‑90 (MQ=255)
           gATGGCGGGGCTGGGGGCGCTGATTGGCAGCGGCGAGGTTAGGTTTTGGGCCGCCCGGGC‑GGCGGGGGTTTTTTGGTGCTTGGTgggggc                                                           >  1:520690/1‑87 (MQ=255)
           gATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGGTAAGTTTTTTGCACGCCTGGCT‑GGGAGGGGTTATTGGGTGCTTGATgggggg                                                           >  2:345338/1‑87 (MQ=255)
                cGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGGTAAGTTTCTGGCACGCCTGGCT‑GGCAGGGGTTATTGGGTGGTTGATGGGGGGCTggg                                                      >  1:166113/1‑89 (MQ=255)
                                          ggcgAGTTAAGTTTCTGGCACGCCTGGCT‑GGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGtttt                            >  2:491350/1‑90 (MQ=255)
                                                              cGCCTGGCT‑GGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTg        <  2:166113/90‑1 (MQ=255)
                                                                    gct‑ggcAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCAtt  >  2:313178/1‑90 (MQ=255)
                                                                             ggATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGc          >  1:524466/1‑74 (MQ=255)
                                                                             ggATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGc          <  2:524466/74‑1 (MQ=255)
                                                                               |                                                                               
GGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCT‑GGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATT  >  NZ_CP009273/67949‑68106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑