Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273262,0780AG54.5% 9.8 / 12.7 11S63P (TCC→CCC) BW25113_RS25425hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base G (6/0);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGG  >  NZ_CP009273/261994‑262152
                                                                                    |                                                                          
gCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACTGCAAAGGGGGGCCGGGTaaat                                                                       >  2:182541/1‑88 (MQ=25)
                     aaCTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAAACTGCAAAGGGGGGCCGGGAAAAAAGGCAACCCGGGACCAttctt                                                  >  2:405272/1‑90 (MQ=255)
                     aaCTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACTGCAAAGGGGGGCCGGGTAAATAATCAAACCGTGACCAttctt                                                  >  2:426214/1‑90 (MQ=255)
                     aaCTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGGAAAAACCGCAAGGGGGGGCCGGGGAAAGAGTCCACCTGTGACCAttctt                                                  >  1:538577/1‑90 (MQ=21)
                     aaCTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGGAAAAACCGCAAGGGGGGACCGGGTAAATAGTCAACCCGTGACCAttttt                                                  >  2:201607/1‑90 (MQ=255)
                         gCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGGAAAAACTGCAAGGGGGGGCCGGGTAAGTAGTCAACCTGGGACCATTCTTCGCt                                              >  2:38772/1‑90 (MQ=255)
                            aTCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGAc                                           <  2:296080/90‑1 (MQ=255)
                                     gCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCtg                                  <  2:110767/90‑1 (MQ=255)
                                               gACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGAcc                        <  1:201607/90‑1 (MQ=255)
                                                                aaCCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCgg       <  1:405272/90‑1 (MQ=255)
                                                                     gCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTgggg  >  2:355149/1‑90 (MQ=255)
                                                                                    |                                                                          
GCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGG  >  NZ_CP009273/261994‑262152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑