Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273646,2650TG53.8% 6.2 / 15.4 13T350P (ACC→CCC) citC[citrate (pro‑3S)‑lyase] ligase
Reads supporting (aligned to +/- strand):  ref base T (0/6);  new base G (7/0);  total (7/6)
Fisher's exact test for biased strand distribution p-value = 5.83e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGC  >  NZ_CP009273/646178‑646326
                                                                                       |                                                             
aTGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTCACCTGTTTTTCATGCGGGGGGCt                                                             >  2:399566/1‑90 (MQ=255)
aTGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTTTTTTTTTCCCTGTTTTTCTTGCGGGGGGCt                                                             >  1:444992/1‑90 (MQ=255)
aTGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTTTCATGCGGGGGGCt                                                             >  2:550715/1‑90 (MQ=255)
    gTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCttttt                                                         >  1:65038/1‑89 (MQ=255)
          tcaCATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTCATTTTTCACCTGTTTTTCATGCGGGGGGCTTTTGACGAGc                                                   >  2:75375/1‑90 (MQ=255)
          tcaCATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCGGGGTTATTTTCCTTTTTTACCTGTTTTTCCTTGGGGGGGCTTTTGACGAGc                                                   >  1:293348/1‑90 (MQ=255)
                        tCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGc                                     <  1:116453/90‑1 (MQ=255)
                         cAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTCATTTTTTACCTGTTTTTCCTGCGGGGGGCTTTTTGCGAGCTGCCGCGTCCTGGCg                                    >  1:293632/1‑90 (MQ=255)
                               tGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTg                              <  2:416764/90‑1 (MQ=255)
                                  cTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTc                           <  1:299078/90‑1 (MQ=255)
                                            gggCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTc                 <  2:50931/90‑1 (MQ=255)
                                                           cATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAGTAATGCAGc  <  1:223234/90‑1 (MQ=255)
                                                           cATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGc  <  1:129107/90‑1 (MQ=255)
                                                                                       |                                                             
ATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGC  >  NZ_CP009273/646178‑646326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑