| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,235,279 | 0 | T | G | 58.3% | 3.4 / 10.1 | 12 | G315G (GGT→GGG) | dadX | catabolic alanine racemase DadX |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (7/0); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTAGTGGACGGCGTGCGCACCATGACGGTGGGGACCGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGTATTGGTACGCCGGTTGAGCTGTGGGGCAAGGAGATCAAAATTGATGATGTCGCCGCCGCTGCCGGAACGGTGGGCTA > NZ_CP009273/1235192‑1235356 | tttAGTGGACGGCGTGCGCACCATGACGGTGGGGACCGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGGAt > 1:247342/1‑90 (MQ=255) ggACGGCGTGCGCACCATGACGGTGGGGACCGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGGGTTGGTat > 1:559741/1‑89 (MQ=255) gACGGCGTGCGCACCATGACGGTGGGGACCGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGTATTGGTACg < 2:128931/90‑1 (MQ=255) gACGGCGTGCGCACCATGACGGTGGGGACCGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGTATTGGTACg < 2:91076/90‑1 (MQ=255) ccATGACGGTGGGGACCGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGTATTGGTACGCCGGTTGAGCtgt < 2:89009/90‑1 (MQ=255) gACGGTGGGGACCGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGTATTGGTACGCCGGTTGAGCTGTgggg < 2:337959/90‑1 (MQ=255) gTGGGGGCCGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGGATTGGTACGCCGGGTGAGCTGTGGGGGAAg > 1:69801/1‑90 (MQ=255) cGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGGATTGGGAAGCCGGGTGGGGTGTGGGGGCAAGAGATCaa > 1:404441/1‑90 (MQ=255) gTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGGATTGGTACGCCGGGTGGGCTGTGGGGCAAGGAGAGCaaa > 1:142967/1‑90 (MQ=255) gCGGTCGATTTAACGCCTTGCCCGCAGGCGGGGATTTGGAAGCCGGTTGGGGTGTGGGGGAAGGAGAACAAAATTTATGATGTtaccgcc > 1:411003/1‑90 (MQ=255) aTTTAACGCCTTGCCCGCAGGCGGGGATTGGTACGCCGGTTGGGCTGTGGGGGAAGGAGATCAAAATTGATGATGTCGCCGCCGCTGCCg > 1:156363/1‑90 (MQ=255) cccGCAGGCGGGTATTGGTCCGCCGGTTGAGCTGTGGGGCAAGGAGATAAAAATTGATGATGTCACCGCCGCTGCCGGAACGGTGGGCTa < 2:411003/90‑1 (MQ=255) | TTTAGTGGACGGCGTGCGCACCATGACGGTGGGGACCGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGTATTGGTACGCCGGTTGAGCTGTGGGGCAAGGAGATCAAAATTGATGATGTCGCCGCCGCTGCCGGAACGGTGGGCTA > NZ_CP009273/1235192‑1235356 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |