Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,737,0170AC54.5% 1.2 / 10.7 11T309P (ACC→CCC) yiaK3‑dehydro‑L‑gulonate 2‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTC  >  NZ_CP009273/3736955‑3737102
                                                              |                                                                                     
ttACTAGGGCCGGGCGGGCTGACAAAAATCAGCCCTTTCGTTTCCCCGCCAATAATTTTACTCCCCTGCGGGCCAAAAACCGCCGTAACg                                                            <  2:270447/90‑1 (MQ=255)
  aCTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGc                                                          >  2:208566/1‑90 (MQ=255)
  aCTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGc                                                          >  2:500687/1‑90 (MQ=255)
    tAGTGCCGGGCGTGCGAAAAAAAACCAGCCCGTCCACCCCCCCGGCTTTAATTTTACTCCCCGGCGGGCAAAAAACCGCCGTAACGGCAt                                                        <  1:456424/90‑1 (MQ=255)
     ggTGCCGGGGGTGCTGAAAAAAACCGGCCCTTCCGCTTCCCCGGCCATAATTTTACTCCCCTGCGGGCCAAAAACCGCCGTAACGGCATc                                                       <  1:171388/89‑1 (MQ=255)
       tGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTCCCCGGCCATGAATTTACTCCCCTGCTGGCCGAAAACCGCCGTAGCGGCATCAc                                                     <  2:18657/90‑1 (MQ=255)
       tGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCAc                                                     >  2:274873/1‑90 (MQ=255)
       ggCCGGGCGTGCGGACAAAAACCCGCCCTTTCGCTTCCCCGGCATTAATTTTCCCCCCCTGCGGGCAAAAACCCGCCGTAACGGCATCAc                                                     <  1:348157/89‑1 (MQ=255)
               gTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTtgatg                                             >  2:140463/1‑90 (MQ=255)
                                                 ccATAAATTTCCTCCCCGGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATgagg           <  2:204328/90‑1 (MQ=255)
                                                      aaTTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATa      >  2:182759/1‑90 (MQ=255)
                                                          tactacCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTc  >  2:22206/1‑90 (MQ=255)
                                                          tactacCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTc  >  2:298249/1‑90 (MQ=255)
                                                              |                                                                                     
TTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTC  >  NZ_CP009273/3736955‑3737102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑