Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,026,0910TG54.5% 9.0 / 12.6 11G80G (GGT→GGG) yigZIMPACT family protein
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base G (6/0);  total (6/5)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGC‑TAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTA  >  NZ_CP009273/4026003‑4026156
                                                                                        |                                                                  
aCCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTa                                                                   <  2:88540/90‑1 (MQ=255)
    ttGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAAcc                                                               >  2:446621/1‑90 (MQ=255)
         tggcgtggGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGc                                                          <  2:460193/90‑1 (MQ=255)
           gcgtggGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTc                                                        <  2:108100/90‑1 (MQ=255)
           gcgtggGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCGATGccc                                                        >  2:33359/1‑88 (MQ=255)
             gtggGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGc                                                      <  1:285806/90‑1 (MQ=255)
                          gcgcCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCCGC‑TAATGGGc                                        >  2:154403/1‑90 (MQ=255)
                                              gCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAAACCATGCCCCCCCCGCTTAAGGGGGGGGGGGGGCGGGGAAATTa                     >  1:470552/1‑90 (MQ=255)
                                                           gacgacGGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCAGC‑TAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTg       >  2:69864/1‑90 (MQ=255)
                                                            acgacgGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGC‑TAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTgc      <  2:43894/90‑1 (MQ=255)
                                                                cgGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCCGC‑TAATGGGGAGCGGCGTCGGGGGAATTACCGCTGTGGGAGGGCGcta  >  2:515951/1‑90 (MQ=255)
                                                                                        |                                                                  
ACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGC‑TAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTA  >  NZ_CP009273/4026003‑4026156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑