Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,571,9400AC54.5% 7.6 / 10.7 11V309G (GTG→GGG) hsdMtype I restriction‑modification system methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base C (0/6);  total (4/7)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATGGTGTGCAGATGACACTTATCCATCAGGTCACGACGAATGTCGGTGCCTTTGCCGCCTTCAAACAGCACGTTATCCGGCACCACCACCGCCGCACGACCGCCGGGATGCAGCGTTTCGATAATATGCTGCATAAAGCACAACTGTTTGTTGCTGGTCGGGTGAACAA  >  NZ_CP009273/4571856‑4572024
                                                                                    |                                                                                    
aTGGTGTGCAGATGACACTTATCCATCAGGTCACGACGAATGTCGGTGCCTTTGCCGCCTTCAAACAGCACGTTATCCGGCaccaccacc                                                                                 <  2:82752/90‑1 (MQ=255)
            tGACACTTATCCATCAGGTCACGACGAATGTCGGTGCCTTTGCCGCCTTCAAACAGCACGTTATCCGGCACCACCACCGCCGCACGAccg                                                                     >  2:474977/1‑90 (MQ=255)
             gACACTTACCCATAAGGTCACGCCGAATGTCGGTGCCTTGGCCGCCTTCAAACAGCACGTTATCCGGCCCCCCCACCGCCGCACGAccgc                                                                    <  1:534889/90‑1 (MQ=255)
               cacTTATCCATCAGGTCACGACGAATGTCGGTGCCTTTGCCGCCTTCAAACAGCACGTTATCCGGCACCCCCACCGCCGCACGAccgccg                                                                  <  1:512190/90‑1 (MQ=255)
                           aGGTCACGTCGAATGTCGGTGCCTTTGCCGCCTTCAAACAGCACGTTATCCGGCACCACCACCGCCGCACGACCGCCGGGATGCAGCGt                                                       >  1:401795/1‑89 (MQ=255)
                           aGGTCACGTCGAATGTCGGTGCCTTTGCCGCCTTCAAACAGCACGTTATCCGGCACCACCACCGCCGCACGACCGCCGGGATGCAGCGt                                                       <  2:401795/89‑1 (MQ=255)
                                 cgacgaATGTCGGTGCCTTTGCCGCCTTCAAACAGCACGTTATCCGGCACCACCACCGCCGCACGACCGCCGGGATGCAGCGTTTCGata                                                >  2:482895/1‑90 (MQ=255)
                                            ggTCCCTTTGCCGCCTTCAAACAGCACTTTACCCGGCCCCCCCCCCGCCGCACGACCGCCGGGATGCAGCGTTTCGATAATATGCTGCAt                                     <  1:307206/90‑1 (MQ=255)
                                                   ttGCCGCCTTCAAACAGCACTTTATCCGGCACCCCCACCGCCGCACGACCGCCGGGATGCAGCGTTTCGATAATATGCTGCATAAAGcac                              <  1:326778/90‑1 (MQ=255)
                                                      ccgctTTAAAACAGCACTTTACCCGGCCCCCCCACCGCCGCCCGACCGCCGGGATGCAGCGTTTCGATAATATGCTGCATAAAGCACAAc                           <  1:474977/90‑1 (MQ=255)
                                                           ttCAAACAGCACGTTATCCGGCCCCCCCACCGCCGCACGACCGCCGGGATGCAGCGTTTCGATAATATGCTGCATAAAGCACAACtgttt                      <  1:259392/90‑1 (MQ=255)
                                                                               gCACCACCACCGCCGCACGACCGCCGGGATGCAGCGTTTCGATAATATGCTGCATAAAGCACAACTGTTTGTTGCTGGTCGGGTGAACaa  >  2:396559/1‑90 (MQ=255)
                                                                                    |                                                                                    
ATGGTGTGCAGATGACACTTATCCATCAGGTCACGACGAATGTCGGTGCCTTTGCCGCCTTCAAACAGCACGTTATCCGGCACCACCACCGCCGCACGACCGCCGGGATGCAGCGTTTCGATAATATGCTGCATAAAGCACAACTGTTTGTTGCTGGTCGGGTGAACAA  >  NZ_CP009273/4571856‑4572024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑