Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	139,618	A→G	E118G (GAA→GGA)	yadG →	ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	139,618	0	A	G	100.0%	62.3 / NA	20	E118G (GAA→GGA)	yadG	ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/7);  total (13/7)

TCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATC  >  NZ_CP009273/139531‑139678                                                                                        |                                                             tCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGgaa                                                            <  1:153896/90‑1 (MQ=255)   aaCCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg                                                          <  1:126269/90‑1 (MQ=255)   aaCCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg                                                          <  1:233970/90‑1 (MQ=255)                  gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg                                                          >  1:120257/1‑75 (MQ=255)                  gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg                                                          <  2:120257/75‑1 (MQ=255)                            ttGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc                                           >  2:373548/1‑80 (MQ=255)                            ttGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc                                           <  1:373548/80‑1 (MQ=255)                            ttGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATggg                                 >  2:377359/1‑90 (MQ=255)                                             gggTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGt                >  1:16615/1‑90 (MQ=255)                                                  ctacGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGcgcg           >  2:122148/1‑90 (MQ=255)                                                   tacGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGt          >  2:559100/1‑90 (MQ=255)                                                       gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATg                                   >  1:272650/1‑61 (MQ=255)                                                       gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATg                                   <  2:272650/61‑1 (MQ=255)                                                       gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt      >  2:280886/1‑90 (MQ=255)                                                       gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt      >  1:125585/1‑90 (MQ=255)                                                         gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAaac                                                >  1:25001/1‑46 (MQ=255)                                                         gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAaac                                                <  2:25001/46‑1 (MQ=255)                                                         gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGcgcg           >  2:194473/1‑83 (MQ=255)                                                         gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGcgcg           <  1:194473/83‑1 (MQ=255)                                                         gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa    >  2:18659/1‑90 (MQ=255)                                                           ggAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTGTGGGGAAAACGCAACGAACGTGCGCGTATGTTATc  >  1:130596/1‑90 (MQ=255)                                                                                        |                                                             TCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATC  >  NZ_CP009273/139531‑139678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑