| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,682,423 | G→C | A71G (GCC→GGC) | fumB ← | class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,682,423 | 0 | G | C | 100.0% | 38.6 / NA | 13 | A71G (GCC→GGC) | fumB | class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (9/4); total (9/4) | |||||||||||
CCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGC > NZ_CP009273/1682336‑1682499 | ccTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGccc > 2:381567/1‑90 (MQ=255) gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCACCTGTTGTTGGTg < 2:120945/90‑1 (MQ=255) tACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGccc > 1:293978/1‑68 (MQ=255) tACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCACCTGTTGTTGGTGCGCCGGAc > 2:130516/1‑90 (MQ=255) tACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCACCTGTTGTTGGTGCGCCGGAc > 2:316951/1‑90 (MQ=255) tACGCAGGAATTGCAGCGCCACACATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGccc < 2:293978/68‑1 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCACCTGTTGTTGGTGCGCCGGACg < 2:181261/90‑1 (MQ=255) tGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAac < 2:428386/90‑1 (MQ=255) gcCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGca > 1:82915/1‑90 (MQ=255) acaTATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAac < 1:189265/81‑1 (MQ=255) acaTATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAac > 2:189265/1‑81 (MQ=255) tATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACg > 2:9886/1‑90 (MQ=255) tttCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCACCTGTTGTTGGTCCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCCGgc > 1:316379/1‑90 (MQ=255) ccGGGTCACGCAGAATGTCGCCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACagag > 1:363879/1‑90 (MQ=255) aCGCAGAATGTCGCCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGc > 1:188697/1‑90 (MQ=255) aCGCAGAATGTCGCCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGc > 2:134405/1‑90 (MQ=255) | CCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGC > NZ_CP009273/1682336‑1682499 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |