Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,012,918	A→G	D351G (GAC→GGC)	fliK →	flagellar hook‑length control protein FliK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,012,918	0	A	G	100.0%	37.4 / NA	13	D351G (GAC→GGC)	fliK	flagellar hook‑length control protein FliK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/5);  total (8/5)

AGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATA  >  NZ_CP009273/2012836‑2012983                                                                                   |                                                                  aGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGGCGACGAt                                                            <  2:343036/90‑1 (MQ=255)             cagcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGGCGACGATACGCTTCCGGtt                                                >  2:517628/1‑90 (MQ=255)                  gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGGCGACGATACGCTTCCGGTTCCCGt                                           >  2:403641/1‑90 (MQ=255)                     ggCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGGCGACGATACGCTTCCGGTTCCCGtctc                                        <  1:365533/90‑1 (MQ=255)                             cccAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGGCGACGATACGCTTCCGGTTCCCGTCTCTTTACAAg                                <  1:403641/90‑1 (MQ=255)                                    acagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGGCGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCgtgt                         >  2:553428/1‑90 (MQ=255)                                         aaaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGGCGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAg                    >  2:534996/1‑90 (MQ=255)                                               aaCGCACAGCAAACCATGAACCTCTGGCGGGGGAAGGCGACGATACGCTTCCGGTTCCCGtc                                          <  1:193379/62‑1 (MQ=255)                                               aaCGCACAGCAAACCATGAACCTCTGGCGGGGGAAGGCGACGATACGCTTCCGGTTCCCGtc                                          >  2:193379/1‑62 (MQ=255)                                                     caGCAAACCATGAACCTCTGGCGGGGGAAGGCGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgcggcg        >  2:503752/1‑90 (MQ=255)                                                           aCCATGAACCTCTGGCGGGGGAAGGCGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGata  >  2:160648/1‑90 (MQ=255)                                                                      ctGGCGGGGGAAGGCGACGATACGCTTCCGGTTCCCGtc                                          >  1:286020/1‑39 (MQ=255)                                                                      ctGGCGGGGGAAGGCGACGATACGCTTCCGGTTCCCGtc                                          <  2:286020/39‑1 (MQ=38)                                                                                   |                                                                  AGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATA  >  NZ_CP009273/2012836‑2012983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑