Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	221,335	T→C	noncoding (1078/1542 nt)	BW25113_RS01010 →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	221,335	0	T	C	100.0%	62.1 / NA	23	noncoding (1078/1542 nt)	BW25113_RS01010	16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/7);  total (16/7)

GACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCA  >  NZ_CP009273/221250‑221420                                                                                      |                                                                                      gACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGaaa                                                                                   >  2:538920/1‑90 (MQ=255)     tCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGtt                                                                               >  2:341161/1‑90 (MQ=255)     tCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGtt                                                                               >  2:149559/1‑90 (MQ=255)           gAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTa                                                                         <  1:149559/90‑1 (MQ=255)                ttCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGt                                                                                >  2:403637/1‑78 (MQ=255)                ttCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGt                                                                                <  1:403637/78‑1 (MQ=255)                ttCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTcc                                                                    >  1:199248/1‑90 (MQ=255)                ttCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTcc                                                                    >  2:14217/1‑90 (MQ=255)                          ggATTGGTGCCTTCGGGAACTGTGAGACCGGTGCTGCATGGCTGTCGTCAGCTCCTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAgc                                                          >  1:435757/1‑90 (MQ=14)                          ggATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAgc                                                          >  1:532396/1‑90 (MQ=255)                               ggTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAAc                                                     >  1:412722/1‑90 (MQ=37)                                  gCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCt                                                  <  2:463421/90‑1 (MQ=18)                                     ttCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTAt                                               >  1:324854/1‑90 (MQ=25)                                                   gaCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGc                                 <  1:380298/90‑1 (MQ=25)                                                       ggTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTc                             >  2:277928/1‑90 (MQ=18)                                                       ggTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTc                             >  1:103555/1‑90 (MQ=18)                                                             gcttGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTccggccg                       >  1:372349/4‑90 (MQ=255)                                                             gcATGGCTGCCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTccggccg                       <  1:497923/90‑1 (MQ=255)                                                                 ggCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGaa                   >  2:282720/1‑90 (MQ=18)                                                                  gCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGGAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAAc                  <  2:435757/90‑1 (MQ=255)                                                                  gCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAAc                  >  1:65759/1‑90 (MQ=18)                                                                            gCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGgaga        >  1:548170/1‑90 (MQ=18)                                                                             cTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGAc       <  1:182178/90‑1 (MQ=18)                                                                                 tgttgcGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGcc   <  2:324854/90‑1 (MQ=18)                                                                                 tgttgcGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGcc   >  2:332908/1‑90 (MQ=18)                                                                                  gttgcGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCa  <  1:180525/90‑1 (MQ=18)                                                                                  gttgcGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCa  <  2:412722/90‑1 (MQ=18)                                                                                      |                                                                                      GACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCA  >  NZ_CP009273/221250‑221420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑