Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,670,359	T→C	G454G (GGA→GGG)	yphE ←	sugar ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,670,359	0	T	C	100.0%	85.9 / NA	27	G454G (GGA→GGG)	yphE	sugar ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/7);  total (20/7)

CGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGAGC  >  NZ_CP009273/2670272‑2670440                                                                                        |                                                                                  cGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTccc                                                                                 <  1:26731/90‑1 (MQ=255)             gtgcTGTAATAACAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGcagccag                                                                     >  2:62653/1‑90 (MQ=255)                 tGTAATAACAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCa                                                                 <  1:19586/90‑1 (MQ=255)                       aaCAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACa                                                           >  2:43162/1‑90 (MQ=255)                         cAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACCCCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAAt                                                         <  2:276715/90‑1 (MQ=255)                            gATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACg                                                               <  2:271378/81‑1 (MQ=255)                            gATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACg                                                               >  1:271378/1‑81 (MQ=255)                            gATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACg                                                      >  2:414216/1‑90 (MQ=255)                            gATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACg                                                      >  2:200399/1‑90 (MQ=255)                               gCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTa                                                   >  1:249846/1‑90 (MQ=255)                                 ggTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTaaa                                                 >  2:319702/1‑90 (MQ=255)                                          ccAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGtt                                        >  1:188430/1‑90 (MQ=255)                                            aGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTg                                      >  2:516070/1‑90 (MQ=255)                                                       ctccACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACgg                                                              <  1:552725/55‑1 (MQ=255)                                                       ctccACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACgg                                                              >  2:552725/1‑55 (MQ=255)                                                           aCTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGAcg                       >  1:318984/1‑90 (MQ=255)                                                            cTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGAcgc                      >  2:320156/1‑90 (MQ=255)                                                              tCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGAcgccg                    <  1:86215/90‑1 (MQ=255)                                                                aCTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAACACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCcgcg                  >  2:295066/1‑90 (MQ=255)                                                                      gaTAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTgg            >  2:94268/1‑90 (MQ=255)                                                                        tAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCt          >  1:122433/1‑90 (MQ=255)                                                                           acacCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCtcgt       >  2:134299/1‑90 (MQ=255)                                                                             accacGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCtt                                  <  2:263619/61‑1 (MQ=255)                                                                             accacGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCtt                                  >  1:263619/1‑61 (MQ=255)                                                                             accacGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCtcgtcg     >  2:275438/1‑90 (MQ=255)                                                                              ccacGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGa    >  2:496843/1‑90 (MQ=255)                                                                                acGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGAGc  >  1:256317/1‑90 (MQ=255)                                                                                acGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGAGc  <  1:200399/90‑1 (MQ=255)                                                                                        |                                                                                  CGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGAGC  >  NZ_CP009273/2670272‑2670440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑