Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,751,237 A→G V241V (GTT→GTC BW25113_RS13685 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,751,2370AG100.0% 59.4 / NA 19V241V (GTT→GTCBW25113_RS13685hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/12);  total (7/12)

CTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCCTTCTTTCCTGCAC  >  NZ_CP009273/2751150‑2751322
                                                                                       |                                                                                     
ctcAAACTCTGACATTTGAATAAAATCCCATAGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGAc                                                                                     <  2:105759/90‑1 (MQ=255)
                tGAATAAAATCCCATAGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGc                                                                     <  1:142630/90‑1 (MQ=255)
                tGAATAAAATCCCATAGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGc                                                                     >  1:395740/1‑90 (MQ=255)
                                    ttCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAAt                                                 <  1:278612/90‑1 (MQ=255)
                                    ttCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAAt                                                 <  2:259619/90‑1 (MQ=255)
                                      cGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGa                                               <  1:207575/90‑1 (MQ=255)
                                            gTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGt                                         <  2:530685/90‑1 (MQ=255)
                                                      tGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCgc                               >  2:225178/1‑90 (MQ=255)
                                                      tGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCgc                               >  2:377830/1‑90 (MQ=255)
                                                      tGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCgc                               >  2:445296/1‑90 (MQ=255)
                                                      tGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAACTCAACCAACTTCAGTTGAATGATATGGTACACTTCCgc                               >  1:311295/1‑90 (MQ=255)
                                                                 tATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGc                    >  2:5228/1‑90 (MQ=255)
                                                                 tATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACCTCCGCTTTGCTTTTGc                    >  2:1069/1‑90 (MQ=255)
                                                                  aTTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGcc                   <  2:324184/90‑1 (MQ=255)
                                                                         cACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCcttc            <  2:551924/90‑1 (MQ=255)
                                                                         cACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCcttc            <  2:535804/90‑1 (MQ=255)
                                                                         cACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCcttc            <  1:153642/90‑1 (MQ=255)
                                                                           cccATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCcttctt          <  2:257022/90‑1 (MQ=255)
                                                                                   tCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCCTTCTTTCCTGCAc  <  1:441125/90‑1 (MQ=255)
                                                                                       |                                                                                     
CTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCCTTCTTTCCTGCAC  >  NZ_CP009273/2751150‑2751322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: