Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,002,903	T→C	S261P (TCA→CCA)	ygeY →	YgeY family selenium metabolism‑linked hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,002,903	0	T	C	100.0%	69.5 / NA	22	S261P (TCA→CCA)	ygeY	YgeY family selenium metabolism‑linked hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/11);  total (11/11)

AAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGA  >  NZ_CP009273/3002829‑3002981                                                                           |                                                                               aaGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCgtcgtc                                                                 <  1:404686/90‑1 (MQ=255)      aCCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGAcc                                                            >  2:465625/1‑90 (MQ=255)             ccGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCg                                                     >  1:359761/1‑90 (MQ=255)              cGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGa                                                    <  1:420799/90‑1 (MQ=255)              cGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGa                                                    <  2:255437/90‑1 (MQ=255)              cGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGa                                                    <  2:187531/90‑1 (MQ=255)                 ttCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAAc                                                 >  1:323678/1‑90 (MQ=255)                   cTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCt                                               >  2:335804/1‑90 (MQ=255)                          cttcttCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAgg                                        >  2:217006/1‑90 (MQ=255)                                cacaTCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGGCCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCt                                  <  2:186966/90‑1 (MQ=255)                                  caTCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTgg                                >  2:162813/1‑90 (MQ=255)                                    tCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGAc                              >  2:130250/1‑90 (MQ=255)                                              gTTGCGCTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCcgcg                    <  2:279304/90‑1 (MQ=255)                                                   gcTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCcctg               <  1:130250/90‑1 (MQ=255)                                                   gcTGTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCcctg               <  1:79791/90‑1 (MQ=255)                                                      gTAGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCcctgcct            >  2:88036/1‑90 (MQ=255)                                                        aGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCctgcctgc          <  2:323678/90‑1 (MQ=255)                                                        aGCAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCctgcctgc          <  2:359761/90‑1 (MQ=255)                                                          cAGACAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAg        <  1:372648/90‑1 (MQ=255)                                                              cAGCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACa    >  1:347120/1‑90 (MQ=255)                                                                gCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGa  >  1:442179/1‑90 (MQ=255)                                                                gCTGCGCAGTTCCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGa  >  2:463764/1‑90 (MQ=255)                                                                           |                                                                               AAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGA  >  NZ_CP009273/3002829‑3002981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑