Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,785,687	G→C	G75G (GGC→GGG)	kbl ←	glycine C‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,785,687	0	G	C	100.0%	81.8 / NA	26	G75G (GGC→GGG)	kbl	glycine C‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (15/11);  total (15/11)

CCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGCACAA  >  NZ_CP009273/3785611‑3785771                                                                             |                                                                                     ccAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTgag                                                                         >  1:97067/1‑90 (MQ=255) ccAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTgag                                                                         <  2:303760/90‑1 (MQ=255)   aGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGaa                                                                       >  2:476658/1‑90 (MQ=255)       aGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCa                                                                   >  2:512630/1‑90 (MQ=255)                    ttGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTgc                                                      <  2:501354/90‑1 (MQ=255)                         cAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCCCCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGccgccg                                                 <  1:21578/90‑1 (MQ=255)                              tctTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCCCCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatc                                            <  1:256696/90‑1 (MQ=255)                              tctTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatc                                            <  1:79243/90‑1 (MQ=255)                              tctTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatc                                            <  2:329423/90‑1 (MQ=255)                                   aTGGCTGTCCTGAGTGCCGCAAATAAAACGCCCCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatcagatc                                       <  1:383156/90‑1 (MQ=255)                                   aTGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatcagatc                                       <  1:383097/90‑1 (MQ=255)                                         gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                                 >  2:1943/1‑90 (MQ=255)                                         gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                                 >  2:464236/1‑90 (MQ=255)                                         gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                                 >  2:443428/1‑90 (MQ=255)                                         gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                                 >  2:375503/1‑90 (MQ=255)                                         gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                                 >  1:210312/1‑90 (MQ=255)                                         gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                                 >  1:555035/1‑90 (MQ=255)                                         gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                                 >  1:24770/1‑90 (MQ=255)                                              gAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGGCTTTGCCGCCGCAATCAGATCAGGATGATTCg                            >  2:479945/1‑90 (MQ=255)                                                   ccGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGc                       >  2:450065/1‑90 (MQ=255)                                                   ccGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGc                       <  1:375503/90‑1 (MQ=255)                                                   ccGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGc                       >  2:545411/1‑90 (MQ=255)                                                   ccGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGc                       >  2:6026/1‑90 (MQ=255)                                                     gCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGccc                     <  1:450065/90‑1 (MQ=255)                                                                 cACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAgttgtt         >  1:452922/1‑90 (MQ=255)                                                                        gCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGCACaa  <  2:18437/90‑1 (MQ=255)                                                                             |                                                                                     CCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGCACAA  >  NZ_CP009273/3785611‑3785771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑