Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,108,830	A→G	H143H (CAT→CAC)	rraA ←	ribonuclease E activity regulator RraA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,108,830	0	A	G	100.0%	38.4 / NA	13	H143H (CAT→CAC)	rraA	ribonuclease E activity regulator RraA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/8);  total (5/8)

AAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAA  >  NZ_CP009273/4108746‑4108888                                                                                     |                                                           aaaaGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGGTGGTc                                                       <  2:230333/90‑1 (MQ=255)        aCCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGGTGGTCGCCGgag                                                >  1:445953/1‑90 (MQ=255)            tGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGGATTGTCGGCATAAAGGAGGTCGACGGagaaga                                            >  2:284030/1‑90 (MQ=255)                                     atCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGGTGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGAcgcg                   <  1:142532/90‑1 (MQ=255)                                        cAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGGTGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGAc                >  1:501722/1‑90 (MQ=255)                                         aGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGGTGGTc                                                       <  1:31636/50‑1 (MQ=255)                                         aGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGGTGGTc                                                       >  2:31636/1‑50 (MQ=255)                                          gCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGGTGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACAt              <  1:58758/90‑1 (MQ=255)                                          gCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGGTGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACAt              <  2:462020/90‑1 (MQ=255)                                                     gAAAGAATAATCCCGGTATTGTCGGCATAAAGGTGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCa   >  2:56384/1‑90 (MQ=255)                                                      aaaGAATAATCCCGGTATTGTCGGCATAAAGGTGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCaa  <  1:232058/90‑1 (MQ=255)                                                      aaaGAATAATCCCGGTATTGTCGGCATAAAGGTGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCaa  <  1:34796/90‑1 (MQ=255)                                                      aaaGAATAATCCCGGTATTGTCGGCATAAAGGTGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCaa  <  2:313009/90‑1 (MQ=255)                                                                                     |                                                           AAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAA  >  NZ_CP009273/4108746‑4108888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑