Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	987,365	A→G	N430D (AAT→GAT)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	987,365	0	A	G	100.0%	34.9 / NA	12	N430D (AAT→GAT)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/9);  total (3/9)

AAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCG  >  NZ_CP009273/987281‑987445                                                                                     |                                                                                 aaaGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTc                                                                             <  1:39059/90‑1 (MQ=255)                      gAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGt                                                        <  1:1109/90‑1 (MQ=255)                      gAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGt                                                        <  2:102488/90‑1 (MQ=255)                      gAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGt                                                        >  2:152312/1‑90 (MQ=255)                                atgGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGcc                                              <  1:328060/90‑1 (MQ=255)                                  ggTAGTGCAGCGCCCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAg                                            <  1:348997/90‑1 (MQ=255)                                  ggTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAg                                            <  2:388790/90‑1 (MQ=255)                                     aGTGCAGCGACCTGTGACGACTTTGGGCAGGCGATTGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGCTGGTACAGCCAGTcc                                         >  1:323960/1‑90 (MQ=255)                                        gCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGt                                      <  2:273441/90‑1 (MQ=255)                                            cGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTacac                                  <  1:61805/90‑1 (MQ=255)                                            cGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTacac                                  <  2:272538/90‑1 (MQ=255)                                                                            gATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCg  >  1:291807/1‑90 (MQ=255)                                                                                     |                                                                                 AAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCG  >  NZ_CP009273/987281‑987445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑