| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 98,192 | T→C | I314T (ATT→ACT) | murC → | UDP‑N‑acetylmuramate‑‑L‑alanine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 98,192 | 0 | T | C | 85.7% | 27.1 / ‑0.2 | 14 | I314T (ATT→ACT) | murC | UDP‑N‑acetylmuramate‑‑L‑alanine ligase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (11/1); total (12/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
CGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAG > NZ_CP009273/98114‑98266 | cgcCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGgcgc > 1:143441/1‑90 (MQ=255) ccAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCtt > 2:26028/1‑90 (MQ=255) ggTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGaa > 2:562263/1‑90 (MQ=255) tcATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCtt > 2:304775/1‑90 (MQ=255) gAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTgg > 2:184960/1‑90 (MQ=255) cAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGtt > 2:489263/1‑90 (MQ=255) aGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGttt < 1:304775/90‑1 (MQ=255) gcggttgcggttGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGgc > 1:259795/1‑53 (MQ=255) gcggttgcggttGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGgc < 2:259795/53‑1 (MQ=255) gcTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGaa > 1:52927/1‑90 (MQ=255) gggCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGGCTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGa > 1:88902/1‑90 (MQ=255) gggCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGa > 2:194011/1‑90 (MQ=255) ttGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAg > 1:248179/1‑90 (MQ=255) ttGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAg > 2:18336/1‑90 (MQ=255) | CGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAG > NZ_CP009273/98114‑98266 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |