Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 108,362 A→G F242S (TTC→TCC)  zapD ← cell division protein ZapD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273108,3620AG100.0% 85.1 / NA 27F242S (TTC→TCC) zapDcell division protein ZapD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/9);  total (18/9)

ACCCCACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTG  >  NZ_CP009273/108277‑108447
                                                                                     |                                                                                      
aCCCCACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATc                                                                                    >  2:293437/1‑90 (MQ=255)
aCCCCACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATc                                                                                    >  1:502027/1‑90 (MQ=255)
      caccacCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACg                                                                              >  1:303584/1‑90 (MQ=255)
          accGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTcc                                                                          >  2:489307/1‑90 (MQ=255)
          accGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTcc                                                                          <  2:409767/90‑1 (MQ=255)
             gTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGt                                                                       >  1:494292/1‑90 (MQ=255)
                    cGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTc                                                               <  2:499515/90‑1 (MQ=255)
                        ggTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGtt                                                           <  2:291882/90‑1 (MQ=255)
                            gggCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGACCTGGTCGGttttag                                                       >  2:319416/1‑88 (MQ=255)
                             ggCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAg                                                      <  2:303584/90‑1 (MQ=255)
                                   tCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCa                                                >  2:59671/1‑90 (MQ=255)
                                      ccGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGt                                                                >  1:394515/1‑71 (MQ=255)
                                      ccGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGt                                                                <  2:394515/71‑1 (MQ=255)
                                           aTAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATa                                        >  2:531789/1‑90 (MQ=255)
                                                ttCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACg                                   >  1:561638/1‑90 (MQ=255)
                                                ttCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACg                                   >  1:92174/1‑90 (MQ=255)
                                                ttCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACg                                   >  1:564402/1‑90 (MQ=255)
                                                           ttACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAAc                        <  2:494292/90‑1 (MQ=255)
                                                           ttACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAAc                        <  2:564402/90‑1 (MQ=255)
                                                                  ttAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCtt                 >  2:361845/1‑90 (MQ=255)
                                                                   tAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGctctca                >  1:322172/1‑88 (MQ=255)
                                                                        aCAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTcc           >  1:205641/1‑90 (MQ=255)
                                                                          aGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCgg         >  1:497184/1‑90 (MQ=255)
                                                                               aGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAtt    >  2:204602/1‑90 (MQ=255)
                                                                                gTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAg                                                      <  1:413048/39‑1 (MQ=255)
                                                                                gTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAg                                                      >  2:413048/1‑39 (MQ=255)
                                                                                 ttCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTg  <  1:544577/90‑1 (MQ=255)
                                                                                     |                                                                                      
ACCCCACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTG  >  NZ_CP009273/108277‑108447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: