Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,331,016	A→G	Y310C (TAT→TGT)	acnA →	aconitate hydratase AcnA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,331,016	0	A	G	100.0%	24.8 / NA	9	Y310C (TAT→TGT)	acnA	aconitate hydratase AcnA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/4);  total (5/4)

TCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGC  >  NZ_CP009273/1330941‑1331077                                                                            |                                                              tcgAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCtgtg                                                 <  1:80260/90‑1 (MQ=255)               gATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATc                                   <  1:21326/90‑1 (MQ=255)               gATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATc                                   >  2:93060/1‑90 (MQ=255)                                      ggCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACAt            >  2:206476/1‑90 (MQ=255)                                          gATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGAt                                >  1:398871/1‑66 (MQ=255)                                          gATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGAt                                <  2:398871/66‑1 (MQ=255)                                          gATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGt        >  2:454216/1‑90 (MQ=255)                                                gcCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGc  >  1:52554/1‑90 (MQ=255)                                                gcCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGc  <  2:52554/90‑1 (MQ=255)                                                                            |                                                              TCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGC  >  NZ_CP009273/1330941‑1331077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑