Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,336,374	T→G	F66C (TTT→TGT)	pyrF →	orotidine‑5'‑phosphate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,336,374	0	T	G	100.0%	27.7 / NA	10	F66C (TTT→TGT)	pyrF	orotidine‑5'‑phosphate decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (4/6);  total (4/6)

GTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGT  >  NZ_CP009273/1336302‑1336455                                                                         |                                                                                  gtcTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTGTGATATCTTTCTTGAcc                                                                  >  2:495466/1‑90 (MQ=255)  tcTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCt                                                                 <  1:41031/90‑1 (MQ=255)              cAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCTGAAATTCCACGa                                                     <  2:286836/90‑1 (MQ=255)                aaGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCTGAAATTCCACGata                                                   <  1:277518/90‑1 (MQ=255)                aaGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCTGAAATTCCACGata                                                   <  1:67686/90‑1 (MQ=255)                            cATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCTGAAATTCCACGata                                                   >  1:374305/1‑78 (MQ=255)                            cATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCTGAAATTCCACGata                                                   <  2:374305/78‑1 (MQ=255)                                gTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCTGAAATTCCACGa                                                     <  1:200326/72‑1 (MQ=255)                                gTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCTGAAATTCCACGa                                                     >  2:200326/1‑72 (MQ=255)                                                                 gCGTGGTTGTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCgt  >  2:15650/1‑90 (MQ=255)                                                                         |                                                                                  GTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGT  >  NZ_CP009273/1336302‑1336455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑