Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,394,163	G→A	L111L (CTG→TTG)	ogt ←	methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,394,163	0	G	A	100.0%	48.6 / NA	14	L111L (CTG→TTG)	ogt	methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/8);  total (6/8)

AGGTACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG  >  NZ_CP009273/1394080‑1394234                                                                                    |                                                                        aGGTACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGcc                                                                   >  2:427426/1‑90 (MQ=255)   gTACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGt                                                                 <  1:362375/90‑1 (MQ=255)    tACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTa                                                                <  1:326213/90‑1 (MQ=255)            tGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTa                                                        >  2:424507/1‑90 (MQ=255)             gCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTAc                                                       >  2:502876/1‑90 (MQ=255)                       ttCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAg                                             <  1:74273/90‑1 (MQ=255)                               gTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGt                                     >  2:82056/1‑90 (MQ=255)                                   gCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGt                                 <  1:427426/90‑1 (MQ=255)                                       cACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAgtg                             <  1:314849/90‑1 (MQ=255)                                       cACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAgtg                             <  2:156888/90‑1 (MQ=255)                                                        gcgccAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCgcgc            <  1:502876/90‑1 (MQ=255)                                                                  ggCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGcc                                                  <  1:66463/42‑1 (MQ=255)                                                                  ggCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGcc                                                  >  2:66463/1‑42 (MQ=255)                                                                  ggCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCg  >  2:149335/1‑90 (MQ=255)                                                                                    |                                                                        AGGTACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG  >  NZ_CP009273/1394080‑1394234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑