| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,499,566 | A→G | S258P (TCC→CCC) | ydcO ← | BenE family transporter YdcO |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,499,566 | 0 | A | G | 88.2% | 44.2 / 1.8 | 17 | coding (772/1176 nt) | ydcO | BenE family transporter YdcO |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (8/7); minor base . (1/1); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01 | |||||||||||
TTATCCGGATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGAAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAGCTGCTTTCATTGCTGCGA > NZ_CP009273/1499487‑1499646 | ttATCCGGATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGt > 1:519417/1‑90 (MQ=255) tATCCGGATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGG‑GAAAACCAGTGc > 2:163821/1‑90 (MQ=255) aTCCGGATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGc > 2:7685/1‑90 (MQ=255) gATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGGCGGAATAAACGCCCAAAGGGGGAAAAACCAGTGCCAGCa > 1:464403/1‑90 (MQ=255) gcgcTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATc < 1:396267/90‑1 (MQ=255) cTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAg < 1:518962/90‑1 (MQ=255) ttCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGG‑GAAAACCAGTGCCAGCAATCCAGTa < 1:163821/90‑1 (MQ=255) tCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTa < 1:7685/90‑1 (MQ=255) ttGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAAt > 1:443096/1‑90 (MQ=255) tAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCg < 1:285903/90‑1 (MQ=255) cgcgGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGaa < 1:249001/88‑1 (MQ=255) cgcgGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGaa > 2:249001/1‑88 (MQ=255) gCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGaa < 2:519417/90‑1 (MQ=255) gACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAgctgc > 1:362111/1‑90 (MQ=255) gACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAgctgc > 1:106783/1‑90 (MQ=255) cGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAGCTGCTTTCATTGCTGCGa < 2:372262/90‑1 (MQ=255) cGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAGCTGCTTTCATTGCTGCGa > 2:396508/1‑90 (MQ=255) | TTATCCGGATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGAAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAGCTGCTTTCATTGCTGCGA > NZ_CP009273/1499487‑1499646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |