Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,502,076	A→G	N347D (AAC→GAC)	rlhA →	23S rRNA 5‑hydroxycytidine C2501 synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,502,076	0	A	G	100.0%	31.5 / NA	11	N347D (AAC→GAC)	rlhA	23S rRNA 5‑hydroxycytidine C2501 synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/7);  total (4/7)

GTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTGG  >  NZ_CP009273/1501998‑1502156                                                                               |                                                                                 gTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGAtt                                                                       <  2:363910/90‑1 (MQ=255)  tAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTa                                                                      <  1:39776/90‑1 (MQ=255)                       cGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTc                                                 <  2:306753/90‑1 (MQ=255)                                 tctcGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATAc                                       <  1:417412/90‑1 (MQ=255)                                     gATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTc                                   >  2:8536/1‑90 (MQ=255)                                                   cGAGCCACTGGCAAATGGCGATGGCCCGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGgaga                     <  2:69066/90‑1 (MQ=255)                                                      gCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTc                                                 <  1:535761/59‑1 (MQ=255)                                                      gCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTc                                                 >  2:535761/1‑59 (MQ=255)                                                              cAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTAcc          <  1:8536/90‑1 (MQ=255)                                                                      gATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTgg  >  1:201623/1‑90 (MQ=255)                                                                      gATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTgg  >  2:364982/1‑90 (MQ=255)                                                                               |                                                                                 GTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTGG  >  NZ_CP009273/1501998‑1502156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑