Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,547,935	A→G	V54A (GTA→GCA)	adhP ←	alcohol dehydrogenase AdhP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,547,935	0	A	G	100.0%	50.5 / NA	17	V54A (GTA→GCA)	adhP	alcohol dehydrogenase AdhP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/9);  total (8/9)

ATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAGGG  >  NZ_CP009273/1547862‑1548016                                                                          |                                                                                  aTCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCAcc                                                                   <  2:285209/90‑1 (MQ=255)      cTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAAt                                                              >  2:338717/1‑90 (MQ=255)                   ggTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAAc                                                 >  1:177413/1‑90 (MQ=255)                         acCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAg                                           <  1:149281/90‑1 (MQ=255)                          ccTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGa                                          <  2:207105/90‑1 (MQ=255)                            tGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATc                                        <  2:249793/90‑1 (MQ=255)                             ggACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCg                                       >  1:494298/1‑90 (MQ=255)                              gACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCgg                                      >  1:499028/1‑90 (MQ=255)                                 ccACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTAt                                   <  2:408911/90‑1 (MQ=255)                                  cACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATg                                  >  2:526263/1‑90 (MQ=255)                                      tCTGCCACCACCCCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACAt                              <  1:257623/90‑1 (MQ=255)                                      tCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACAt                              <  2:105214/90‑1 (MQ=255)                                      tCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACAt                              <  1:25170/90‑1 (MQ=255)                                                    gATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTc                >  1:122220/1‑90 (MQ=255)                                                    gATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTc                <  1:338717/90‑1 (MQ=255)                                                          ttCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCAtttt          >  1:282462/1‑90 (MQ=255)                                                                  ccAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAggg  >  1:449951/1‑90 (MQ=255)                                                                          |                                                                                  ATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAGGG  >  NZ_CP009273/1547862‑1548016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑