| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,560,820 | T→C | E193G (GAA→GGA) | dosC ← | diguanylate cyclase DosC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,560,820 | 0 | T | C | 90.9% | 27.9 / ‑3.2 | 11 | E193G (GAA→GGA) | dosC | diguanylate cyclase DosC |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (5/5); minor base A (1/0); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01 | |||||||||||
TAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTTCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCC > NZ_CP009273/1560734‑1560906 | tAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTcccc < 2:221011/90‑1 (MQ=255) cAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTACCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTttc > 1:456338/1‑90 (MQ=255) gCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGc > 1:246308/1‑90 (MQ=255) aGAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAg < 1:159585/90‑1 (MQ=255) gAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGt > 1:142347/1‑90 (MQ=255) gAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTCCTTTTTCTTCTTCGGCGTTTTCCAGt > 2:117798/1‑90 (MQ=255) tCCAGCAGGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAAc < 1:560864/90‑1 (MQ=255) aTTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATa > 2:193511/1‑90 (MQ=255) tataGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGAt < 1:193511/90‑1 (MQ=255) gATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGtt > 2:86587/1‑90 (MQ=255) tCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCt < 1:243447/90‑1 (MQ=255) aTTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTcc < 2:402494/90‑1 (MQ=255) | TAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTTCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCC > NZ_CP009273/1560734‑1560906 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |