Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,568,198	A→G	F417S (TTC→TCC)	pqqL ←	insulinase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,568,198	0	A	G	100.0%	46.0 / NA	15	F417S (TTC→TCC)	pqqL	insulinase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/12);  total (3/12)

AACTGCTGCCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTC  >  NZ_CP009273/1568111‑1568259                                                                                        |                                                              aaCTGCTGCCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGAc                                                             <  1:181326/90‑1 (MQ=255) aaCTGCTGCCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGAc                                                             <  2:490537/90‑1 (MQ=255)   ctgctgCCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACgg                                                           <  1:394282/90‑1 (MQ=255)   ctgctgCCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACgg                                                           <  2:185148/90‑1 (MQ=255)      ctgcCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGt                                                        >  1:383588/1‑90 (MQ=255)       tgcCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGta                                                       <  1:399401/90‑1 (MQ=255)                           ttGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGc                                   >  2:392007/1‑90 (MQ=255)                            tGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGcc                                  <  2:544089/90‑1 (MQ=255)                                  gTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGc                              <  1:4398/88‑1 (MQ=255)                                  gTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGc                              >  2:4398/1‑88 (MQ=255)                                   tAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGAc                           <  1:421180/90‑1 (MQ=255)                                   tAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGAc                           <  2:39668/90‑1 (MQ=255)                                     aTTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTg                         <  2:131983/90‑1 (MQ=255)                                           tgcCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTA‑TTAATGAGCTGGATGCCAGGCGACTGGTCAGCa                  <  1:246053/90‑1 (MQ=255)                                                            aaGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTc  <  2:51528/90‑1 (MQ=255)                                                                                        |                                                              AACTGCTGCCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTC  >  NZ_CP009273/1568111‑1568259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑