Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 120,483 A→C K327T (AAA→ACA)  aceE → pyruvate dehydrogenase (acetyl‑transferring), homodimeric type

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273120,4830AC100.0% 59.2 / NA 19K327T (AAA→ACA) aceEpyruvate dehydrogenase (acetyl‑transferring), homodimeric type
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (8/11);  total (8/11)

TGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGC  >  NZ_CP009273/120398‑120569
                                                                                     |                                                                                      
tGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCg                                                                                    >  1:232360/1‑90 (MQ=255)
tGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCg                                                                                    >  2:502133/1‑90 (MQ=255)
      gAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGAt                                                                              <  1:250343/90‑1 (MQ=255)
      gAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGAt                                                                              <  2:104914/90‑1 (MQ=255)
        aCTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGATgg                                                                            <  2:138491/90‑1 (MQ=255)
           gctgcGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGAt                                                                              <  2:304937/85‑1 (MQ=255)
           gctgcGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGAt                                                                              >  1:304937/1‑85 (MQ=255)
           gctgcGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGATGGTGc                                                                         >  1:118814/1‑90 (MQ=255)
             tgcGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGATGGTGCGt                                                                       <  1:491666/90‑1 (MQ=255)
                gTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGATGGTGCGTACg                                                                    >  2:38378/1‑90 (MQ=255)
                                  aaCTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCg                                                  <  1:38378/90‑1 (MQ=255)
                                        tCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAat                                            <  2:141125/90‑1 (MQ=255)
                                                      gAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGATGGTGCGTAc                                                                     <  1:368049/51‑1 (MQ=255)
                                                      gAAACCGTTGACGGCGACTACCAGACCTTCACATCGAAAGATGGTGCGTAc                                                                     >  2:368049/1‑51 (MQ=255)
                                                                aCGGCGACTACCAGACCTTCACATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTg                    <  2:118814/90‑1 (MQ=255)
                                                                   gCGACTACCAGACCTTCACATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAg                 <  2:26744/90‑1 (MQ=255)
                                                                    cGACTACCAGACCTTCACATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAga                >  2:460322/1‑90 (MQ=255)
                                                                                 ttCACATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAg   >  2:544735/1‑90 (MQ=255)
                                                                                  tCACATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGc  <  2:367728/90‑1 (MQ=255)
                                                                                     |                                                                                      
TGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGC  >  NZ_CP009273/120398‑120569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: