Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,673,542	A→G	K287E (AAG→GAG)	ydgH →	DUF1471 family protein YdgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,673,542	0	A	G	100.0%	32.3 / NA	14	K287E (AAG→GAG)	ydgH	DUF1471 family protein YdgH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/8);  total (6/8)

ATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATC  >  NZ_CP009273/1673458‑1673617                                                                                     |                                                                            atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa                                                                        <  1:180789/90‑1 (MQ=255)           tCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGt                                                              >  2:363788/1‑90 (MQ=255)              aCAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTAtt                                                           <  1:20417/90‑1 (MQ=255)              aCAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTAtt                                                           >  2:500887/1‑90 (MQ=255)                                    cTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCa                                     >  1:431233/1‑90 (MQ=255)                                           aaCATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGGGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGt                              <  2:45577/90‑1 (MQ=255)                                           aaCATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGt                              <  2:105549/90‑1 (MQ=255)                                              aTGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGt                           >  2:35019/1‑90 (MQ=255)                                                           ccTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACCTGGGGATAACCTGGCCg              >  2:412456/1‑90 (MQ=255)                                                             tATCAGGTTGCAAAACGGGCCGGGGAAAAAGGTGCTAATTTTTCCCCCACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc            <  1:156474/90‑1 (MQ=255)                                                             tATCAGGTTGCAAAACGGGCCGGGGAAAAAGGTGCTAATTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc            <  1:35019/90‑1 (MQ=255)                                                                  ggTTGCAAAACGGGCCGCGGAGAAAGGTGAGAAATATTCCCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc       <  1:255615/90‑1 (MQ=255)                                                                     tGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGGGGGAATAACCTGACCGTCAGCGCAGa    >  1:232427/1‑90 (MQ=255)                                                                       cGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATc  <  2:431233/90‑1 (MQ=255)                                                                                     |                                                                            ATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATC  >  NZ_CP009273/1673458‑1673617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑