Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,240,670	Δ1 bp	coding (55/441 nt)	BW25113_RS06225 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,240,667	0	G	.	100.0%	32.7 / NA	9	coding (52/441 nt)	BW25113_RS06225	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (6/3);  total (6/3)

CTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAA  >  NZ_CP009273/1240585‑1240744                                                                                   |                                                                              cTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGAt                                                                       >  2:375273/1‑90 (MQ=255)                              gtgtGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGa                                          <  1:285561/90‑1 (MQ=255)                                 tgATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAgg                                                                <  1:253364/65‑1 (MQ=255)                                 tgATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAgg                                                                >  2:253364/1‑65 (MQ=255)                                        gAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGaa                                >  2:146054/1‑90 (MQ=255)                                                  atTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATc                      <  1:197463/90‑1 (MQ=255)                                                                 cAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTTAAAAATTAAAATTTATCAATCCTTTTGGCAGAttta       >  2:418318/1‑88 (MQ=255)                                                                   gTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTGGAAATTTAGAAATGATTAATCCTTCTTGCAGATTAAGc     >  1:241827/1‑90 (MQ=255)                                                                      aGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGGGGCCCTTTTTGAAAATTTAGAAATTAGCAATCCGTGCGGGCGATTAAGGCaa  >  1:259559/1‑90 (MQ=255)                                                                                   |                                                                              CTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAA  >  NZ_CP009273/1240585‑1240744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑